Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 11864968 
Holland PM, et al. (2002) Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2. J Biol Chem 277, 16229-40 11864968
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

T202-p - ERK1 (human)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HMVEC (endothelial)
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
TNF increase
IL-1a increase

Y204-p - ERK1 (human)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HMVEC (endothelial)
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
TNF increase
IL-1a increase

T185-p - ERK2 (human)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HMVEC (endothelial)
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
TNF increase
IL-1a increase

Y187-p - ERK2 (human)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HMVEC (endothelial)
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
TNF increase
IL-1a increase

T183-p - JNK1 (human)
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HMVEC (endothelial)
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
TNF increase
IL-1a increase

Y185-p - JNK1 (human)
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HMVEC (endothelial)
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
TNF increase
IL-1a increase

T210-p - NEK9 (human)
Orthologous residues
NEK9 (human): T210‑p, NEK9 (mouse): T210‑p, NEK9 (rat): T209‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE NEK9 (human) phosphoaminoacid analysis, mutation in upstream enzyme recognition motif
 Comments:  autophosphorylation

S72-p - CSN2 (cow)
Orthologous residues
CSN2 (human): P63‑p, CSN2 (mouse): P70‑p, CSN2 (pig): P73‑p, CSN2 (cow): S72‑p, CSN2 (goat): S72‑p, CSN2 (horse): P79‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE NEK9 (human)

S139-p - CSN2 (cow)
Orthologous residues
CSN2 (human): I130‑p, CSN2 (mouse): I143‑p, CSN2 (pig): S140‑p, CSN2 (cow): S139‑p, CSN2 (goat): S139‑p, CSN2 (horse): I145‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE NEK9 (human)

S157-p - CSN2 (cow)
Orthologous residues
CSN2 (human): P148‑p, CSN2 (mouse): , CSN2 (pig): S156‑p, CSN2 (cow): S157‑p, CSN2 (goat): S157‑p, CSN2 (horse): P163‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE NEK9 (human)


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.