Curated Information
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Curated Information Page
PubMed Id: 12024051 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Xu X, Tsvetkov LM, Stern DF (2002) Chk2 activation and phosphorylation-dependent oligomerization. Mol Cell Biol 22, 4419-32 12024051
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T26-p - Chk2 (human)
Orthologous residues
Chk2 (human): T26‑p, Chk2 iso12 (human): T26‑p, Chk2 (mouse): S29‑p, Chk2 (rat): S28‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, immunoprecipitation, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), reticulocyte
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rabbit
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
caffeine decrease
wortmannin decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation

S28-p - Chk2 (human)
Orthologous residues
Chk2 (human): S28‑p, Chk2 iso12 (human): S28‑p, Chk2 (mouse): P31‑p, Chk2 (rat): S30‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, immunoprecipitation, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), reticulocyte
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rabbit
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) pharmacological inhibitor of upstream enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
caffeine decrease
wortmannin decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation

T68-p - Chk2 (human)
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, immunoprecipitation, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), reticulocyte
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rabbit
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation


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