Curated Information
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Curated Information Page
PubMed Id: 11390408 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Lee CH, Chung JH (2001) The hCds1 (Chk2)-FHA domain is essential for a chain of phosphorylation events on hCds1 that is induced by ionizing radiation. J Biol Chem 276, 30537-41 11390408
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T68-p - Chk2 (human)
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic transfer of constitutively active upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation
 Comments:  phosphorylation of this site is required for subsequent phosphorylation of T387 and T383

T383-p - Chk2 (human)
Orthologous residues
Chk2 (human): T383‑p, Chk2 iso12 (human): T354‑p, Chk2 (mouse): T387‑p, Chk2 (rat): T386‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Chk2 (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

T387-p - Chk2 (human)
Orthologous residues
Chk2 (human): T387‑p, Chk2 iso12 (human): T358‑p, Chk2 (mouse): T391‑p, Chk2 (rat): T390‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Chk2 (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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