Curated Information

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PubMed Id: 19553453

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus^® and may be incomplete.

Shi Y, et al. (2009) Focal adhesion kinase acts downstream of EphB receptors to maintain mature dendritic spines by regulating cofilin activity. J Neurosci 29, 8129-42 19553453

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

S3-p - cofilin 1 (mouse)

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Orthologous residues

cofilin 1 (human): S3‑p, cofilin 1 (mouse): S3‑p, cofilin 1 (rat): S3‑p, cofilin 1 (pig): S3‑p

Characterization

Methods used to characterize site in vivo: mutation of modification site, phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 'neuron, hippocampal'-'brain, embryonic'

Cellular systems studied: primary cells

Species studied: mouse

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
ephrin B2				increase
Y27632	ephrin B2			inhibit treatment-induced increase

Downstream Regulation

Effect of modification (process): cytoskeletal reorganization

Comments: S3 phosphorylation is mediated by FAK and regulates morphology of dendritic spines.

Y428-p - FAK (mouse)

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Orthologous residues

FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y428‑p, FAK iso3 (mouse): Y397‑p, FAK iso4 (mouse): Y397‑p, FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken): ‑

Characterization

Methods used to characterize site in vivo: mutation of modification site, phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 'neuron, hippocampal'-'brain, embryonic'

Cellular systems studied: primary cells

Species studied: mouse

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
ephrin B2				increase

Downstream Regulation

Effect of modification (function): enzymatic activity, induced

Effect of modification (process): cytoskeletal reorganization, signaling pathway regulation

Comments: regulates dendritic spine length and morphology; activate RhoA-ROCK-LIMK pathway

T508-p - LIMK1 (mouse)

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Orthologous residues

LIMK1 (human): T508‑p, LIMK1 (mouse): T508‑p, LIMK1 (rat): T508‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 'neuron, hippocampal'-'brain, embryonic'

Cellular systems studied: primary cells

Species studied: mouse

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
EphB2				increase

T505-p - LIMK2 (mouse)

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Orthologous residues

LIMK2 (human): T505‑p, LIMK2 iso2 (human): T484‑p, LIMK2 (mouse): T505‑p, LIMK2 (rat): T505‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 'neuron, hippocampal'-'brain, embryonic'

Cellular systems studied: primary cells

Species studied: mouse

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
EphB2				increase

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