Curated Information
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PubMed Id: 19287004 
Yu L, et al. (2009) JAK2 and SHP2 Reciprocally Regulate Tyrosine Phosphorylation and Stability of Proapoptotic Protein ASK1. J Biol Chem 284, 13481-8 19287004
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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Y718-p - ASK1 (human)
Modsite: IPERDSRySQPLHEE SwissProt Entrez-Gene
Orthologous residues
ASK1 (human): Y718‑p, ASK1 iso5 (human): Y798‑p, ASK1 (mouse): Y725‑p, ASK1 (rat): Y724‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (human) activation of upstream enzyme, transfection of wild-type enzyme, co-immunoprecipitation, phospho-antibody, mutation in upstream enzyme recognition motif
PHOSPHATASE SHP-2 (human) transfection of inactive enzyme, transfection of wild-type enzyme, co-immunoprecipitation
Comments:  "substrate trapping" mutant
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-beta no change compared to control
TNF no change compared to control
IFN-gamma increase
IFN-gamma SOCS1 (human) no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein degradation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SOCS1 (human) Induces molecular association, regulation co-immunoprecipitation
Comments:  SHP2 dephosphorylation of ASK1 Y798 disrupts binding of ASK1 to SOCS1 and induces apoptosis.

T838-p - ASK1 (human)
Modsite: GINPCTEtFTGTLQY SwissProt Entrez-Gene
Orthologous residues
ASK1 (human): T838‑p, ASK1 iso5 (human): T918‑p, ASK1 (mouse): T845‑p, ASK1 (rat): T844‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF SHP-2 (human) increase

Y1007-p - JAK2 (human)
Modsite: VLPQDKEyYKVKEPG SwissProt Entrez-Gene
Orthologous residues
JAK2 (human): Y1007‑p, JAK2 (mouse): Y1007‑p, JAK2 (rat): Y1007‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase
IFN-gamma increase
IFN-gamma SHP-2 (human) no effect upon treatment-induced increase

Y1008-p - JAK2 (human)
Modsite: LPQDKEYyKVKEPGE SwissProt Entrez-Gene
Orthologous residues
JAK2 (human): Y1008‑p, JAK2 (mouse): Y1008‑p, JAK2 (rat): Y1008‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase
IFN-gamma increase
IFN-gamma SHP-2 (human) no effect upon treatment-induced increase

T183-p - JNK1 (human)
Modsite: AGTSFMMtPYVVTRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p

Y185-p - JNK1 (human)
Modsite: TSFMMTPyVVTRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p

T183-p - JNK2 (human)
Modsite: ACTNFMMtPYVVTRY SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): T183‑p, JNK2 iso2 (human): T183‑p, JNK2 iso3 (human): T183‑p, JNK2 (mouse): T183‑p, JNK2 (rat): T183‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF SHP-2 (human) increase

Y185-p - JNK2 (human)
Modsite: TNFMMTPyVVTRYYR SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): Y185‑p, JNK2 iso2 (human): Y185‑p, JNK2 iso3 (human): Y185‑p, JNK2 (mouse): Y185‑p, JNK2 (rat): Y185‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF SHP-2 (human) increase

Y546-p - SHP-2 (human)
Modsite: SKRKGHEyTNIKYSL SwissProt Entrez-Gene
Orthologous residues
SHP‑2 (human): Y546‑p, SHP‑2 iso2 (human): Y542‑p, SHP‑2 (mouse): Y546‑p, SHP‑2 (rat): Y546‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF SHP-2 (human) increase

Y701-p - STAT1 (human)
Modsite: DGPKGTGyIKTELIS SwissProt Entrez-Gene
Orthologous residues
STAT1 (human): Y701‑p, STAT1 iso2 (human): Y701‑p, STAT1 (mouse): Y701‑p, STAT1 (rat): Y701‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase


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