Curated Information
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Curated Information Page
PubMed Id: 18347057 
Bayascas JR, et al. (2008) Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance. Mol Cell Biol 28, 3258-72 18347057
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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T308-p - Akt1 (mouse)
Modsite: KDGATMKtFCGTPEY SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, AKT1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, AKT1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T203-p - ERK1 (mouse)
Modsite: HDHTGFLtEYVATRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

Y205-p - ERK1 (mouse)
Modsite: HTGFLTEyVATRWYR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T183-p - ERK2 (mouse)
Modsite: HDHTGFLtEYVATRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

Y185-p - ERK2 (mouse)
Modsite: HTGFLTEyVATRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

S253-p - FOXO1A (mouse)
Modsite: SPRRRAAsMDNNSKF SwissProt Entrez-Gene
Orthologous residues
FOXO1A (human): S256‑p, FOXO1A (mouse): S253‑p, FOXO1A (rat): S250‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)

S21-p - GSK3A (mouse)
Modsite: SGRARTSsFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

S9-p - GSK3B (mouse)
Modsite: SGRPRTTsFAESCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S3‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

Y1179-p - INSR (mouse)
Modsite: RDIYETDyYRKGGKG SwissProt Entrez-Gene
Orthologous residues
INSR (human): Y1189‑p, INSR iso2 (human): Y1177‑p, INSR (mouse): Y1179‑p, INSR (rat): Y1190‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

Y1180-p - INSR (mouse)
Modsite: DIYETDYyRKGGKGL SwissProt Entrez-Gene
Orthologous residues
INSR (human): Y1190‑p, INSR iso2 (human): Y1178‑p, INSR (mouse): Y1180‑p, INSR (rat): Y1191‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T346-p - NDRG1 (mouse)
Modsite: GTRSRSHtSEGPRSR SwissProt Entrez-Gene
Orthologous residues
NDRG1 (human): T346‑p, NDRG1 iso2 (human): T280‑p, NDRG1 iso3 (human): T265‑p, NDRG1 (mouse): T346‑p, NDRG1 (rat): T346‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T356-p - NDRG1 (mouse)
Modsite: GPRSRSHtSEGSRSR SwissProt Entrez-Gene
Orthologous residues
NDRG1 (human): T356‑p, NDRG1 iso2 (human): T290‑p, NDRG1 iso3 (human): T275‑p, NDRG1 (mouse): T356‑p, NDRG1 (rat): T356‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T366-p - NDRG1 (mouse)
Modsite: GSRSRSHtSEDARLN SwissProt Entrez-Gene
Orthologous residues
NDRG1 (human): T366‑p, NDRG1 iso2 (human): T300‑p, NDRG1 iso3 (human): T285‑p, NDRG1 (mouse): T366‑p, NDRG1 (rat): T366‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T252-p - p70S6K (mouse)
Modsite: HDGTVTHtFCGTIEY SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T252‑p, p70S6K iso2 (human): T229‑p, p70S6K (mouse): T252‑p, p70S6K (rat): T252‑p, p70S6K iso2 (rat): T229‑p, p70S6K (fruit fly): T238‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)
meal feeding increase
meal feeding PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T412-p - p70S6K (mouse)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)
meal feeding increase
meal feeding PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T247-p - PRAS40 (mouse)
Modsite: LPRPRLNtSDFQKLK SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)

S235-p - S6 (mouse)
Modsite: IAKRRRLsSLRASTS SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)
meal feeding increase
meal feeding PDK1 (mouse) no effect upon treatment-induced increase dominant negative (PH domain mutant)

T1465-p - TSC2 (mouse)
Modsite: GLRPRGYtISDSAPS SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso2 (human): T1419‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 'stem, embryonic', adipose tissue, heart, liver
Cellular systems studied:  primary cultured cells, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PDK1 (mouse) inhibit treatment-induced increase dominant negative (PH domain mutant)


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