Curated Information
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Curated Information Page
PubMed Id:  
Possemato A (2007) CST Curation Set: 2284; Year: 2007; Biosample/Treatment: cell line, Jurkat/calyculin &'||' pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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Y850-p - AKAP2 (human)
Modsite: LRWEAGIyANQEEED SwissProt Entrez-Gene
Orthologous residues
AKAP2 (human): Y850‑p, AKAP2 iso4 (human): Y1094‑p, AKAP2 (mouse): Y884‑p, AKAP2 iso3 (mouse): , AKAP2 iso6 (mouse): Y912‑p, AKAP2 (rat): Y861‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y239-p - CD7 (human)
Modsite: TLSSPNQyQ______ SwissProt Entrez-Gene
Orthologous residues
CD7 (human): Y239‑p, CD7 (mouse): Y209‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y127-p - EIF5A (human)
Modsite: GKEIEQKyDCGEEIL SwissProt Entrez-Gene
Orthologous residues
EIF5A (human): Y127‑p, EIF5A iso2 (human): Y157‑p, EIF5A (mouse): Y127‑p, EIF5A (rat): Y127‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y85-p - emerin (human)
Modsite: KKEDALLyQSKGYND SwissProt Entrez-Gene
Orthologous residues
emerin (human): Y85‑p, emerin (mouse): Y86‑p, emerin (rat): Y86‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y94-p - emerin (human)
Modsite: SKGYNDDyYEESYFT SwissProt Entrez-Gene
Orthologous residues
emerin (human): Y94‑p, emerin (mouse): Y95‑p, emerin (rat): Y95‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y95-p - emerin (human)
Modsite: KGYNDDYyEESYFTT SwissProt Entrez-Gene
Orthologous residues
emerin (human): Y95‑p, emerin (mouse): Y96‑p, emerin (rat): Y96‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y107-p - IL32 (human)
Modsite: YLETVAAyYEEQHPE SwissProt Entrez-Gene
Orthologous residues
IL32 (human): Y107‑p, IL32 iso2 (human): Y61‑p, IL32 iso4 (human): Y61‑p, IL32 iso5 (human): Y41‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y108-p - IL32 (human)
Modsite: LETVAAYyEEQHPEL SwissProt Entrez-Gene
Orthologous residues
IL32 (human): Y108‑p, IL32 iso2 (human): Y62‑p, IL32 iso4 (human): Y62‑p, IL32 iso5 (human): Y42‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y66-p - KPNA4 (human)
Modsite: DSDIDGDyRVQNTSL SwissProt Entrez-Gene
Orthologous residues
KPNA4 (human): Y66‑p, KPNA4 (mouse): Y66‑p, KPNA4 (rat): Y66‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y194-p - PIH1D1 (human)
Modsite: IQELGDLyTPAPGRA SwissProt Entrez-Gene
Orthologous residues
PIH1D1 (human): Y194‑p, PIH1D1 iso2 (human): , PIH1D1 (mouse): D194‑p, PIH1D1 (rat): D194‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y256-p - RPS3A (human)
Modsite: KVERADGyEPPVQES SwissProt Entrez-Gene
Orthologous residues
RPS3A (human): Y256‑p, RPS3A (mouse): Y256‑p, RPS3A (rat): Y256‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y116-p - SLY (human)
Modsite: ASPTSPDySLDSPGP SwissProt Entrez-Gene
Orthologous residues
SLY (human): Y116‑p, SLY (mouse): C116‑p, SLY (rat): C116‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y187-p - THUMPD3 (human)
Modsite: LDSHILDyYENPAIK SwissProt Entrez-Gene
Orthologous residues
THUMPD3 (human): Y187‑p, THUMPD3 (mouse): Y189‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y1903-p - TPR (human)
Modsite: EGVTQGDyTPMEDSE SwissProt Entrez-Gene
Orthologous residues
TPR (human): Y1903‑p, TPR (mouse): Y1973‑p, TPR (rat): Y1902‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y422-p - TUBB (human)
Modsite: MNDLVSEyQQYQDAT SwissProt Entrez-Gene
Orthologous residues
TUBB (human): Y422‑p, TUBB (mouse): Y422‑p, TUBB (rat): Y422‑p, TUBB (cow): Y422‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y425-p - TUBB (human)
Modsite: LVSEYQQyQDATAEE SwissProt Entrez-Gene
Orthologous residues
TUBB (human): Y425‑p, TUBB (mouse): Y425‑p, TUBB (rat): Y425‑p, TUBB (cow): Y425‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y219-p - UFD1L (human)
Modsite: GEADHSGyAGELGFR SwissProt Entrez-Gene
Orthologous residues
UFD1L (human): Y219‑p, UFD1L (mouse): Y219‑p, UFD1L (rat): Y219‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S87-p - UPRT (human)
Modsite: SGSGDSSsYDAPAGN SwissProt Entrez-Gene
Orthologous residues
UPRT (human): S87‑p, UPRT (mouse): N87‑p, UPRT (rat): N87‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y455-p - ZW10 (human)
Modsite: QKVSNTQyHEVMNLE SwissProt Entrez-Gene
Orthologous residues
ZW10 (human): Y455‑p, ZW10 (mouse): F455‑p, ZW10 (rat): F453‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human


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