Curated Information
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Curated Information Page
PubMed Id:  
Possemato A (2008) CST Curation Set: 4085; Year: 2008; Biosample/Treatment: cell line, Jurkat/pervanadate &'||' calyculin; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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Y209-p - CD28 (human)
Modsite: TRKHYQPyAPPRDFA SwissProt Entrez-Gene
Orthologous residues
CD28 (human): Y209‑p, CD28 (mouse): Y207‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y123-p - CD3Z (human)
Modsite: KDKMAEAySEIGMKG SwissProt Entrez-Gene
Orthologous residues
CD3Z (human): Y123‑p, CD3Z iso3 (human): Y122‑p, CD3Z (mouse): Y123‑p, CD3Z iso2 (mouse): Y123‑p, CD3Z (rat): Y123‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y222-p - CD7 (human)
Modsite: NSAACVVyEDMSHSR SwissProt Entrez-Gene
Orthologous residues
CD7 (human): Y222‑p, CD7 (mouse): Y192‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y520-p - CPSF6 (human)
Modsite: SRDRHDDyYRERSRE SwissProt Entrez-Gene
Orthologous residues
CPSF6 (human): Y520‑p, CPSF6 iso2 (human): Y557‑p, CPSF6 (mouse): Y520‑p, CPSF6 (rat): Y520‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y381-p - DNAJA1 (human)
Modsite: RHYNGEAyEDDEHHP SwissProt Entrez-Gene
Orthologous residues
DNAJA1 (human): Y381‑p, DNAJA1 (mouse): Y381‑p, DNAJA1 (rat): Y381‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y209-p - DOCK2 (human)
Modsite: MSKDQPDyAMYSRIS SwissProt Entrez-Gene
Orthologous residues
DOCK2 (human): Y209‑p, DOCK2 (mouse): Y209‑p, DOCK2 (rat): Y209‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y212-p - DOCK2 (human)
Modsite: DQPDYAMySRISSSP SwissProt Entrez-Gene
Orthologous residues
DOCK2 (human): Y212‑p, DOCK2 (mouse): Y212‑p, DOCK2 (rat): Y212‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y316-p - EIF4B (human)
Modsite: SWSSRDDySRDDYRR SwissProt Entrez-Gene
Orthologous residues
EIF4B (human): Y316‑p, EIF4B (mouse): Y316‑p, EIF4B (rat): Y316‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y1042-p - Erbin (human)
Modsite: NVRANTAyHLHQRLG SwissProt Entrez-Gene
Orthologous residues
Erbin (human): Y1042‑p, Erbin iso8 (human): Y1042‑p, Erbin (mouse): Y1040‑p, Erbin iso2 (mouse): Y1040‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y279-p - GSK3A (human)
Modsite: RGEPNVSyICSRYYR SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): Y279‑p, GSK3A (mouse): Y279‑p, GSK3A (rat): Y279‑p, GSK3A (cow): Y279‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y216-p - GSK3B (human)
Modsite: RGEPNVSyICSRYYR SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): Y216‑p, GSK3B iso2 (human): Y216‑p, GSK3B (mouse): Y216‑p, GSK3B (rat): Y216‑p, GSK3B (rabbit):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y296-p - hnRNP 2H9 (human)
Modsite: GMDNQGGyGSVGRMG SwissProt Entrez-Gene
Orthologous residues
hnRNP 2H9 (human): Y296‑p, hnRNP 2H9 iso2 (human): Y281‑p, hnRNP 2H9 iso3 (human): Y247‑p, hnRNP 2H9 (mouse): Y296‑p, hnRNP 2H9 (rat): Y296‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y235-p - hnRNP A/B (human)
Modsite: VAQPKEVyQQQQYGS SwissProt Entrez-Gene
Orthologous residues
hnRNP A/B (human): Y235‑p, hnRNP A/B iso2 (human): Y235‑p, hnRNP A/B iso3 (human): Y235‑p, hnRNP A/B (mouse): Y240‑p, hnRNP A/B iso2 (mouse): Y233‑p, hnRNP A/B (rat): Y239‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y834-p - NICE-4 (human)
Modsite: QTRFPLDyYSIPFPT SwissProt Entrez-Gene
Orthologous residues
NICE‑4 (human): Y834‑p, NICE‑4 iso2 (human): Y834‑p, NICE‑4 iso5 (human): Y834‑p, NICE‑4 (mouse): Y854‑p, NICE‑4 iso2 (mouse): Y834‑p, NICE‑4 (rat): Y854‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y460-p - OTUD4 (human)
Modsite: IEESRLLyEIQNRDE SwissProt Entrez-Gene
Orthologous residues
OTUD4 (human): Y460‑p, OTUD4 iso3 (human): Y395‑p, OTUD4 (mouse): Y459‑p, OTUD4 (rat): Y458‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y1772-p - PIKFYVE (human)
Modsite: LRGADSAyYQVGQTG SwissProt Entrez-Gene
Orthologous residues
PIKFYVE (human): Y1772‑p, PIKFYVE iso2 (human): , PIKFYVE iso6 (human): , PIKFYVE (mouse): Y1771‑p, PIKFYVE iso1 (mouse): Y1726‑p, PIKFYVE iso3 (mouse): , PIKFYVE (rat): Y1726‑p, PIKFYVE iso2 (rat): Y1771‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y64-p - PPP1R11 (human)
Modsite: SSKCCCIyEKPRAFG SwissProt Entrez-Gene
Orthologous residues
PPP1R11 (human): Y64‑p, PPP1R11 (mouse): Y69‑p, PPP1R11 (rat): Y65‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y84-p - SMAP (human)
Modsite: NEELESQyQQSMDSK SwissProt Entrez-Gene
Orthologous residues
SMAP (human): Y84‑p, SMAP (mouse): Y84‑p, SMAP (rat): Y84‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y371-p - STAM2 (human)
Modsite: LVNEAPVySVYSKLH SwissProt Entrez-Gene
Orthologous residues
STAM2 (human): Y371‑p, STAM2 iso2 (human): , STAM2 (mouse): Y371‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y426-p - ZO2 (human)
Modsite: RRHQYSDyDYHSSSE SwissProt Entrez-Gene
Orthologous residues
ZO2 (human): Y426‑p, ZO2 iso2 (human): Y426‑p, ZO2 iso3 (human): Y403‑p, ZO2 (mouse): Q406‑p, ZO2 (rat): Q406‑p, ZO2 (dog): Y410‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y428-p - ZO2 (human)
Modsite: HQYSDYDyHSSSEKL SwissProt Entrez-Gene
Orthologous residues
ZO2 (human): Y428‑p, ZO2 iso2 (human): Y428‑p, ZO2 iso3 (human): Y405‑p, ZO2 (mouse): Y408‑p, ZO2 (rat): Y408‑p, ZO2 (dog): Y412‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human


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