Taniguchi T, et al. (2001) Phosphorylation of tau is regulated by PKN. J Biol Chem 276, 10025-31 11104762

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T181-p - Tau iso8 (human) ▲

Modsite: ktPPAPktPPssGEP SwissProt Entrez-Gene Orthologous residues Tau (human): T498‑p, Tau iso2 (human): T123‑p, Tau iso3 (human): T87‑p, Tau iso4 (human): T152‑p, Tau iso5 (human): T181‑p, Tau iso6 (human): T123‑p, Tau iso7 (human): T152‑p, Tau iso8 (human): T181‑p, Tau (mouse): T473‑p, Tau iso3 (mouse): T170‑p, Tau iso7 (mouse): T112‑p, Tau (rat): T492‑p, Tau (cow): T172‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SK-N-MC (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PKN1 (human) decrease activated

S202-p - Tau iso8 (human) ▲

Modsite: sGyssPGsPGtPGsR SwissProt Entrez-Gene Orthologous residues Tau (human): S519‑p, Tau iso2 (human): S144‑p, Tau iso3 (human): S108‑p, Tau iso4 (human): S173‑p, Tau iso5 (human): S202‑p, Tau iso6 (human): S144‑p, Tau iso7 (human): S173‑p, Tau iso8 (human): S202‑p, Tau (mouse): S494‑p, Tau iso3 (mouse): S191‑p, Tau iso7 (mouse): S151‑p, Tau (rat): S513‑p, Tau (cow): S209‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SK-N-MC (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PKN1 (human) decrease activated

T205-p - Tau iso8 (human) ▲

Modsite: ssPGsPGtPGsRsRt SwissProt Entrez-Gene Orthologous residues Tau (human): T522‑p, Tau iso2 (human): T147‑p, Tau iso3 (human): T111‑p, Tau iso4 (human): T176‑p, Tau iso5 (human): T205‑p, Tau iso6 (human): T147‑p, Tau iso7 (human): T176‑p, Tau iso8 (human): T205‑p, Tau (mouse): T497‑p, Tau iso3 (mouse): T194‑p, Tau iso7 (mouse): T154‑p, Tau (rat): T516‑p, Tau (cow): T212‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SK-N-MC (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PKN1 (human) decrease activated

S214-p - Tau iso8 (human) ▲

Modsite: GsRsRtPsLPtPPtR SwissProt Entrez-Gene Orthologous residues Tau (human): S531‑p, Tau iso2 (human): S156‑p, Tau iso3 (human): S120‑p, Tau iso4 (human): S185‑p, Tau iso5 (human): S214‑p, Tau iso6 (human): S156‑p, Tau iso7 (human): S185‑p, Tau iso8 (human): S214‑p, Tau (mouse): S506‑p, Tau iso3 (mouse): S203‑p, Tau iso7 (mouse): S163‑p, Tau (rat): S525‑p, Tau (cow): S221‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKN1 (human)

T231-p - Tau iso8 (human) ▲

Modsite: KKVAVVRtPPKsPss SwissProt Entrez-Gene Orthologous residues Tau (human): T548‑p, Tau iso2 (human): T173‑p, Tau iso3 (human): T137‑p, Tau iso4 (human): T202‑p, Tau iso5 (human): T231‑p, Tau iso6 (human): T173‑p, Tau iso7 (human): T202‑p, Tau iso8 (human): T231‑p, Tau (mouse): T523‑p, Tau iso3 (mouse): T220‑p, Tau iso7 (mouse): T180‑p, Tau (rat): T542‑p, Tau (cow): T238‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SK-N-MC (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PKN1 (human) decrease activated

S258-p - Tau iso8 (human) ▲

Modsite: PDLkNVKskIGstEN SwissProt Entrez-Gene Orthologous residues Tau (human): S575‑p, Tau iso2 (human): S200‑p, Tau iso3 (human): S164‑p, Tau iso4 (human): S229‑p, Tau iso5 (human): S258‑p, Tau iso6 (human): S200‑p, Tau iso7 (human): S229‑p, Tau iso8 (human): S258‑p, Tau (mouse): S550‑p, Tau iso3 (mouse): S247‑p, Tau iso7 (mouse): S207‑p, Tau (rat): S569‑p, Tau (cow): S265‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCA (human) KINASE PKCE (human) KINASE PKN1 (human) KINASE PKCB iso2 (human) KINASE PKCZ (human) KINASE PKCG (human) KINASE PKCI (human) KINASE PKCB (human)

S293-p - Tau iso8 (human) ▲

Modsite: NVQskCGsKDNIkHV SwissProt Entrez-Gene Orthologous residues Tau (human): S610‑p, Tau iso2 (human): ‑, Tau iso3 (human): ‑, Tau iso4 (human): ‑, Tau iso5 (human): ‑, Tau iso6 (human): S235‑p, Tau iso7 (human): S264‑p, Tau iso8 (human): S293‑p, Tau (mouse): S585‑p, Tau iso3 (mouse): S282‑p, Tau iso7 (mouse): S242‑p, Tau (rat): S604‑p, Tau (cow): S300‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCE (human) KINASE PKCG (human) KINASE PKCB iso2 (human) KINASE PKCI (human) KINASE PKCZ (human) KINASE PKCA (human) KINASE PKCB (human)

S320-p - Tau iso8 (human) ▲

Modsite: VDLskVtskCGsLGN SwissProt Entrez-Gene Orthologous residues Tau (human): S637‑p, Tau iso2 (human): S231‑p, Tau iso3 (human): S195‑p, Tau iso4 (human): S260‑p, Tau iso5 (human): S289‑p, Tau iso6 (human): S262‑p, Tau iso7 (human): S291‑p, Tau iso8 (human): S320‑p, Tau (mouse): S612‑p, Tau iso3 (mouse): S309‑p, Tau iso7 (mouse): S269‑p, Tau (rat): S631‑p, Tau (cow): S327‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  CHO (fibroblast), SK-N-MC (neural crest) Cellular systems studied:  cell lines Species studied:  hamster, human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKN1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKN1 (human) transfection of constitutively active enzyme, phospho-antibody PHOSPHATASE PPP3CA (human) pharmacological inhibitor of upstream enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes FK506 increase vanadate increase okadaic_acid increase

S324-p - Tau iso8 (human) ▲

Modsite: kVtskCGsLGNIHHk SwissProt Entrez-Gene Orthologous residues Tau (human): S641‑p, Tau iso2 (human): S235‑p, Tau iso3 (human): S199‑p, Tau iso4 (human): S264‑p, Tau iso5 (human): S293‑p, Tau iso6 (human): S266‑p, Tau iso7 (human): S295‑p, Tau iso8 (human): S324‑p, Tau (mouse): S616‑p, Tau iso3 (mouse): S313‑p, Tau iso7 (mouse): S273‑p, Tau (rat): S635‑p, Tau (cow): S331‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCB iso2 (human) KINASE PKCG (human) KINASE PKCB (human) KINASE PKCE (human) KINASE PKCZ (human) KINASE PKCA (human) KINASE PKCI (human)

S352-p - Tau iso8 (human) ▲

Modsite: DFKDrVQskIGsLDN SwissProt Entrez-Gene Orthologous residues Tau (human): S669‑p, Tau iso2 (human): S263‑p, Tau iso3 (human): S227‑p, Tau iso4 (human): S292‑p, Tau iso5 (human): S321‑p, Tau iso6 (human): S294‑p, Tau iso7 (human): S323‑p, Tau iso8 (human): S352‑p, Tau (mouse): S644‑p, Tau iso3 (mouse): S341‑p, Tau iso7 (mouse): S301‑p, Tau (rat): S663‑p, Tau (cow): S359‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCZ (human) KINASE PKCA (human) KINASE PKCB iso2 (human) KINASE PKCB (human) KINASE PKCI (human) KINASE PKN1 (human) KINASE PKCE (human) KINASE PKCG (human)