Nguyen H, Ramana CV, Bayes J, Stark GR (2001) Roles of phosphatidylinositol 3-kinase in interferon-gamma-dependent phosphorylation of STAT1 on serine 727 and activation of gene expression. J Biol Chem 276, 33361-8 11438544

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S473-p - Akt1 (human) ▲

Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  2C4 (fibroblast), 2fTGH (fibroblast), HeLa (cervical), T98G (glial), U4A (fibroblast) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase

T202-p - ERK1 (human) ▲

Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  2C4 (fibroblast), 2fTGH (fibroblast), B9 (fibroblast), U4A (fibroblast) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase

Y204-p - ERK1 (human) ▲

Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene Orthologous residues ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  2C4 (fibroblast), 2fTGH (fibroblast), B9 (fibroblast), U4A (fibroblast) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase

T185-p - ERK2 (human) ▲

Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  2C4 (fibroblast), 2fTGH (fibroblast), B9 (fibroblast), U4A (fibroblast) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase

Y187-p - ERK2 (human) ▲

Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene Orthologous residues ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  2C4 (fibroblast), 2fTGH (fibroblast), B9 (fibroblast), U4A (fibroblast) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase

Y701-p - STAT1 (human) ▲

Modsite: DGPkGtGyIktELIs SwissProt Entrez-Gene Orthologous residues STAT1 (human): Y701‑p, STAT1 iso2 (human): Y701‑p, STAT1 (mouse): Y701‑p, STAT1 (rat): Y701‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, phospho-antibody Relevant cell lines - cell types - tissues:  2C4 (fibroblast), 2fTGH (fibroblast), T98G (glial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE JAK2 (human) transfection of wild-type enzyme KINASE JAK1 (human) transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase LY294002 IFN-gamma no effect upon treatment-induced increase

S727-p - STAT1 (human) ▲

Modsite: TDNLLPMsPEEFDEV SwissProt Entrez-Gene Orthologous residues STAT1 (human): S727‑p, STAT1 iso2 (human): ‑, STAT1 (mouse): S727‑p, STAT1 (rat): S727‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, phospho-antibody Relevant cell lines - cell types - tissues:  2C4 (fibroblast), 2fTGH (fibroblast), T98G (glial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase LY294002 IFN-gamma inhibit treatment-induced increase TNF increase LY294002 TNF no effect upon treatment-induced increase IL-1b increase LY294002 IL-1b no effect upon treatment-induced increase Downstream Regulation Effect of modification (function):  activity, induced Effect of modification (process):  transcription, induced

S473-p - Akt1 (mouse) ▲

Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  SCC16-5 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase

T203-p - ERK1 (mouse) ▲

Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  SCC16-5 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase IFN-gamma increase

T183-p - ERK2 (mouse) ▲

Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  SCC16-5 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase

Y185-p - ERK2 (mouse) ▲

Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene Orthologous residues ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  SCC16-5 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase

Y701-p - STAT1 (mouse) ▲

Modsite: DDPKRTGyIkTELIs SwissProt Entrez-Gene Orthologous residues STAT1 (human): Y701‑p, STAT1 iso2 (human): Y701‑p, STAT1 (mouse): Y701‑p, STAT1 (rat): Y701‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  SCC16-5 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase

S727-p - STAT1 (mouse) ▲

Modsite: tDNLLPMsPEEFDEM SwissProt Entrez-Gene Orthologous residues STAT1 (human): S727‑p, STAT1 iso2 (human): ‑, STAT1 (mouse): S727‑p, STAT1 (rat): S727‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  SCC16-5 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IFN-gamma increase